| Overview |
| bs-70064R |
| DGCR8 (Ser377) Antibody |
| WB |
| Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Human, Mouse |
| Specifications |
| Unconjugated |
| Rabbit |
| Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH). |
| Ser377 |
| Polyclonal |
| IgG |
| Lot Dependent |
| Antigen Affinity purification from Pooled whole antiserum |
| 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
| Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Target |
| 1644 |
| Q8WYQ5 |
| DGCRK6 antibody, C22orf12 antibody, D16H22S788E antibody, D16Wis2 antibody, DGCR 8 antibody, Dgcr8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8_HUMAN antibody, DGCRK 6 antibody, DiGeorge syndrome critical region 8 antibody, DiGeorge syndrome critical region gene 8 antibody, Gy1 antibody, Microprocessor complex subunit DGCR8 antibody, pasha antibody |
| The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010). |
| Application Dilution |
| WB |
1:300-5000 |
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