| Overview |
| bs-70166CH |
| Myelin Basic Protein (MBP) Antibody |
| WB, IHC, ICC |
| Specific for endogenous levels of the ~18 kDa & ~22 kDa myelin basic protein. |
| Human, Mouse, Rat, Bovine |
| Specifications |
| Unconjugated |
| Chicken |
| Three peptide sequences conserved in higher vertebrate MBP proteins |
| Polyclonal |
| IgY |
| Lot Dependent |
| Total IgY fraction |
| PBS + 10 mM NaN3. |
| Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. |
| Target |
| 24547 |
| P02688 |
| GDB antibody, Golli MBP antibody, Golli MBP myelin basic protein antibody, Hemopoietic MBP antibody, HMBPR antibody, HUGO antibody, MBP antibody, MBP_CAVPO antibody, MBP_HUMAN antibody, MGC99675 antibody, MLD antibody, Myelin A1 protein antibody, Myelin A1 Protein basic antibody, Myelin basic protein antibody, Myelin Deficient antibody, Myelin membrane encephalitogenic protein antibody, OTTHUMP00000163776 antibodym, OTTHUMP00000174387 antibody, OTTHUMP00000174388 antibody, SHI antibody, Shiverer antibody, SP antibody |
| Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa. |
| Application Dilution |
| WB |
1:300-5000 |
| IHC |
|
| ICC |
1:100-500 |
Powered by Bioz