| Overview |
| bs-70666R |
| SMAD7 (N-terminal region) Antibody |
| WB |
| This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry. |
| Human, Mouse, Rat, Xenopus |
| Specifications |
| Unconjugated |
| Rabbit |
| This antibody was raised against amino acids from the N-terminal region of human SMAD7. This sequence is 100% or highly conserved between human and multiple species. |
| Polyclonal |
| IgG |
| Purified by Protein G. |
| PBS + 0.05% BSA and 0.05% NaN3 |
| Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. |
| Target |
| O15105 |
| MADH7, MADH8, MAD7 |
| SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. |
| Application Dilution |
| WB |
1:300-5000 |
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