_-Tubulin (Ser-172), Phosphospecific Antibody

Applications

Reactivity

Human
Mouse
Rat
Chicken

Overview
Catalog #	bs-70711r
Product Name	_-Tubulin (Ser-172), Phosphospecific Antibody
Applications	WB
Specificity	This antibody was cross-adsorbed to unphosphorylated _III-Tubulin (Ser-172) peptide before affinity purification using phospho-_III-Tubulin (Ser-172) peptide (without carrier). The antibody detects a 50 kDa* protein corresponding to the molecular mass of _-Tubulin on SDS-PAGE immunoblots of purified brain tubulin phosphorylated with ERK2 kinase. This sequence is identical to similar regions in _I, _II, and _III-Tubulin isotypes, and is well conserved in tubulins from most eukaryotic species.
Reactivity	Human, Mouse, Rat, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Phospho-_III-Tubulin (Ser-172) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 172 of human _III-Tubulin.
Modification Site	Ser-172
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Purification	Antigen Affinity purification
Storage Buffer	PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Storage Condition	Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C.
Target
Swiss Prot	Q13509
Synonyms	TUBB3
Background	Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of _/_-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in _-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of _III-Tubulin in vitro. Unphosphorylated Ser-444 in _III-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in _-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.
Application Dilution
WB	1:300-5000