| Overview |
| bs-70725r |
| N-WASP Antibody |
| WB |
| This antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP on SDS-PAGE immunoblots of neonatal rat brain lysate. It is also detects 65 kDa* proteins in A431, human endothelial, and SKN-SH cells. It does not recognize the 63 kDa* WASP protein in Jurkat cell lysate. This N-WASP peptide sequence is 100% homologous to rat and mouse N-WASP, and has low homology to the corresponding region in the human WASP. |
| Human, Mouse, Rat |
| Specifications |
| Unconjugated |
| Rabbit |
| N-WASP synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human N-WASP. |
| Polyclonal |
| #REF! |
| IgG |
| Antigen Affinity purification |
| PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage at -20C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20C. |
| Target |
| O00401 |
| Neural Wiskott-Aldrich syndrome protein, WASL, WASP |
| Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain. |
| Application Dilution |
| WB |
1:300-5000 |
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