TOMM20 Polyclonal Antibody

$Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Brain tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Kidney tissue lysates; Lane 6: Rat Brain tissue lysates; Lane 7: Rat Heart tissue lysates; Lane 8: Human Hela cell lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human HL-60 cell lysates; Lane 11: Human 293T cell lysates probed with TOMM20 Polyclonal Antibody, Unconjugated (bs-7357R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Kidney tissue lysates; Lane 2: Mouse Brain tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse NIH\/3T3 cell lysates; Lane 5: Rat Kidney tissue lysates; Lane 6: Rat Brain tissue lysates; Lane 7: Rat Heart tissue lysates; Lane 8: Human Hela cell lysates; Lane 9: Human THP-1 cell lysates; Lane 10: Human HL-60 cell lysates; Lane 11: Human 293T cell lysates probed with TOMM20 Polyclonal Antibody, Unconjugated (bs-7357R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-7357r
Product Name	TOMM20 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human TOM20
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9804
Swiss Prot	Q15388
Subcellular location	Cytoplasm
Synonyms	TOM20; KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM 20; TOM20CHUMAN; Translocase of outer mitochondrial membrane 20 homolog (yeast); Translocase of outer mitochondrial membrane 20 homolog type II.
Background	Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
Application Dilution
WB	=1:500-2000
ELISA	ELISA=1:5000-10000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500