PGA3 Polyclonal Antibody

$Lane 1: Mouse Stomach tissue lysates; Lane 2: Rat Stomach tissue lysates; Lane 3: Human MKN45 cell lysates probed with PGA3 Polyclonal Antibody, Unconjugated (bs-7369R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Stomach tissue lysates; Lane 2: Rat Stomach tissue lysates; Lane 3: Human MKN45 cell lysates probed with PGA3 Polyclonal Antibody, Unconjugated (bs-7369R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-7369R
Product Name	PGA3 Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Pepsinogen I
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	643834
Swiss Prot	P0DJD8
Synonyms	PEPA3_HUMAN; PGI; Pepsin A-3; Pepsinogen 3 group I; PEPSINOGEN I--SECOND LOCUS; Pepsinogen-3; PGA3.
Background	Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity, and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The amino acid composition of Pepsin C differs from those of pepsinogen and pepsin especially in the content of basic amino acids, glutamic acid, aspartic acid, leucine and isoleucine.
Application Dilution
WB
ELISA	IHC-P1:200-400
IHC-P	IF
IHC-F
IF
ICC/IF