beta Defensin 3 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:400 dilution, for 1.5 hours at 37\u00b0C.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:400 dilution, for 1.5 hours at 37\u00b0C.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:100 dilution, for 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation: Anti-beta Defensin 3 Antibody, conjugated (bs-7378R-FITC) at a 1:100 dilution, for 1.5 hours at 37\u00b0C; DAPI (5ug\/ml, blue) was used to stain the cell nuclei.

$Blank control cells (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3 FITC Conjugated antibody (bs-7378R-FITC; green), Dilution: 1\u03bcg in 100 \u03bcL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG FITC Conjugated (orange), used under the same conditions.$

Blank control cells (blue): Hep G2 Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-beta Defensin 3 FITC Conjugated antibody (bs-7378R-FITC; green), Dilution: 1\u03bcg in 100 \u03bcL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG FITC Conjugated (orange), used under the same conditions.

Applications

ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
ICC

Reactivity

Human

Overview
Catalog #	bs-7378R
Product Name	beta Defensin 3 Polyclonal Antibody
Applications	ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human beta Defensin 3
Immunogen Range	23-67/67
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	414325
Swiss Prot	P81534
Subcellular location	Secreted
Synonyms	BD 3; BD-3; BD3; beta 103; Beta defensin 103A; Beta defensin 103A precursor; Beta defensin 3; Beta-defensin 103; Beta-defensin 3; D103A_HUMAN; DEF B3; DEFB 103; DEFB 103A; DEFB 3; DEFB-3; DEFB103; DEFB103A; DEFB103B; DEFB3; Defensin; Defensin beta 103A; Defensin beta 3; Defensin like protein; Defensin-like protein; HBD 3; hBD-3; HBD3; HBP 3; HBP3; mBD3.
Background	Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
Application Dilution
ELISA	1:500-1000
FCM	1:20-100
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200
ICC	1:100-500