Luciferase Polyclonal Antibody, Biotin Conjugated

Applications

  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Tags
  • Others
Overview
Catalog # bs-8539R-Biotin
Product Name Luciferase Polyclonal Antibody, Biotin Conjugated
Applications ELISA, IHC-P, IHC-F
Reactivity Tags, Others
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from Firefly Luciferase
Immunogen Range 101-220/550
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 7100
Subcellular location Cytoplasm
Synonyms Luciferin 4-monooxygenase
Background Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Application Dilution
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500