IL-6 (2C6) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-6 (2C6) Monoclonal Antibody, Unconjugated (bsm-10807M) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with IL-6 (2C6) Monoclonal Antibody, Unconjugated (bsm-10807M) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: Recombinant human IL-6 protein, C-His (bs-10807P); Lane 2: Recombinant human IL-6 protein, C-His (bs-41177P) probed with human IL-6 Monoclonal Antibody, Unconjugated (bsm-10807M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Recombinant human IL-6 protein, C-His (bs-10807P); Lane 2: Recombinant human IL-6 protein, C-His (bs-41177P) probed with human IL-6 Monoclonal Antibody, Unconjugated (bsm-10807M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human

Overview
Catalog #	bsm-10807M
Product Name	IL-6 (2C6) Monoclonal Antibody
Applications	IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human IL-6 corresponding to 30-212/212aa (C-6x His-Tag)
Clonality	Monoclonal
Clone #	2C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	3569
Swiss Prot	P05231
Synonyms	Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin-6; Interleukin6; IL6_HUMAN.
Background	This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF

Applications

Reactivity

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