PGC Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (Human Fundus of stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-25702M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (Human Fundus of stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-25702M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Cow
Sheep
Rabbit

Overview
Catalog #	bsm-25702m
Product Name	PGC Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Cow, Sheep, Rabbit
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human PGC
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5225
Swiss Prot	P20142
Subcellular location	Secreted
Synonyms	PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin;
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF