V5 tag Monoclonal Antibody

$8ug Tags Positive Control Whole Cell Lysate, His (bs-41230P) per lane probed with V5 tag monoclonal antibody respectively, unconjugated (bsm-33005M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.$

8ug Tags Positive Control Whole Cell Lysate, His (bs-41230P) per lane probed with V5 tag monoclonal antibody respectively, unconjugated (bsm-33005M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
ELISA

Reactivity

Species independent

Overview
Catalog #	bsm-33005m
Product Name	V5 tag Monoclonal Antibody
Applications	WB, ELISA
Reactivity	Species independent
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated V5-tag peptide
Clonality	Monoclonal
Clone #	5D7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms	V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag.
Background	The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Application Dilution
WB	1:300-5000
ELISA	1:500-1000