SGK1 Monoclonal Antibody

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human Siha cell lysates; Lane 5: Human HUVEC cell lysates probed with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse RAW264.7 cell lysates; Lane 3: Human Hela cell lysates; Lane 4: Human Siha cell lysates; Lane 5: Human HUVEC cell lysates probed with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:200 overnight at 4\u00b0C, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat bladder; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SGK1 Monoclonal Antibody, Unconjugated (bsm-33275M) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF(ICC)
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-33275M
Product Name	SGK1 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF(ICC), IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human SGK1
Clonality	Monoclonal
Clone #	5.00E+11
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein G.
Storage Buffer	Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6446
Swiss Prot	O00141
Synonyms	Serum/glucocorticoid regulated kinase 1; SGK 1; SGK-1; Serine/threonine protein kinase SGK; Serine/threonine protein kinase Sgk1; Serum and glucocorticoid regulated kinase; Serum/glucocorticoid regulated kinase; SGK 1; SGK; SGK1_HUMAN.
Background	SGK1 is a protein kinase that plays an important role in cellular stress response. SGK1 activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels of SGK1 and activity may contribute to conditions such as hypertension and diabetic nephropathy. This protein also mediates cell survival signals, as it has been shown to phosphorylate and negatively regulate the pro apoptotic FOXO3A protein. Ser 422 is a critical site on the protein and may be involved in its activation.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF(ICC)	1:50-200
IF

Applications

Reactivity

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