P63 Monoclonal Antibody

Blank control\uff08black line\uff09:A549. \r\n \r\nPrimary Antibody (green line): Mouse Anti-P63 antibody (bsm-33417M) \r\n\r\nDilution:1ug\/Test; \r\n \r\nSecondary Antibody\uff08white blue line\uff09: Goat anti-Mouse IgG-AF488\r\n\r\nDilution: 0.5ug\/Test. \r\n\r\nIsotype control\uff08orange line\uff09: Normal mouse IgG\r\n\r\nProtocol\r\n\r\nThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.