BOB1 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded \tHuman Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded \tHuman Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded \tHuman Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded \tHuman Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded \tHuman Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded \tHuman Lymph; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BOB1 Monoclonal Antibody, Unconjugated(bsm-34182M) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Mouse, sp-0024) and DAB (C-0010) staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Human

Overview
Catalog #	bsm-34182m
Product Name	BOB1 Monoclonal Antibody
Applications	IHC-P, IHC-F, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human BOb-1
Clonality	Monoclonal
Clone #	5G9
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5450
Swiss Prot	Q16633
Subcellular location	Nucleus
Synonyms	B-cell oct-binding protein 1; B-cell-specific coactivator OBF-1; POU domain class 2-associating factor 1; POU2AF1; OCT-binding factor 1; BOB-1; OCA-B. OBF1; OCA-B; OBF1_HUMAN; BOB.1.
Application Dilution
IHC-P	1:200-400
IHC-F	1:100-500
IF