PGC Monoclonal Antibody

$Lane 1: Recombinant human Pepsinogen C \/ PGC protein, His(bs-41229P) probed with PGC Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.$

Lane 1: Recombinant human Pepsinogen C \/ PGC protein, His(bs-41229P) probed with PGC Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.

$Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

$Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (PGC) Monoclonal Antibody, Unconjugated (bsm-41229M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-41229m
Product Name	PGC Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human Pepsinogen II
Clonality	Monoclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5225
Swiss Prot	P20142
Subcellular location	Secreted
Synonyms	PEPC_HUMAN; Gastricsin; EC:3.4.23.3; Pepsinogen C; PEPC; PGII; progastricsin; Pepsinogen II;
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF