beta-Amyloid (1-42) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Amyloid) Monoclonal Antibody, Unconjugated (bsm-41470M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n$

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (beta-Amyloid) Monoclonal Antibody, Unconjugated (bsm-41470M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.\r\n\r\n

$Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-41470M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining. \r\n\r\n$

Positive sample\uff08left\uff09\uff1aAlzheimer's Disease Brain\r\nNegative sample\uff08right\uff09\uff1aNormal Human Brain\r\nBlock endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Incubation with (beta-Amyloid(1-42)) Monoclona Antibody, Unconjugated (bsm-41470M) at 1:1000 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0024) instructionsand DAB staining. \r\n\r\n

$100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-41470M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.$

100 ng A\u03b21-42 Peptide (bs-0107P) per lane probed with beta-Amyloid(1-42) polyclonal antibody respectively, unconjugated (bsm-41470M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-41470m
Product Name	beta-Amyloid (1-42) Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human beta-Amyloid(1-42)
Clonality	Monoclonal
Clone #	6C8
Isotype	IgG2b
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	351
Swiss Prot	P05067
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF