C-jun Monoclonal Antibody

$Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Recombinant human C-jun protein(50ng) probed with C-jun Monoclonal Antibody, Unconjugated (bsm-42050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human U87MG cell lysates; Lane 2: Human U251 cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Recombinant human C-jun protein(50ng) probed with C-jun Monoclonal Antibody, Unconjugated (bsm-42050M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (C-jun) polyclonal Antibody, Unconjugated (bsm-42050M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.$

Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37\u00b0C for 20 min; Antibody incubation with (C-jun) polyclonal Antibody, Unconjugated (bsm-42050M) 1:100, 90 minutes at 37\u00b0C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37\u00b0C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-42050M
Product Name	C-jun Monoclonal Antibody
Applications	WB
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human C-jun protein
Clonality	Monoclonal
Clone #	-
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	3725
Swiss Prot	P05412
Subcellular location	Nucleus
Synonyms	Transcription factor AP-1; Jun oncogene; JUN; AP 1; AP1; AP-1; Enhancer Binding Protein AP1; Jun Activation Domain Binding Protein; JUN protein; JUNC; p39; Proto oncogene cJun; Transcription Factor AP1; V jun avian sarcoma virus 17 oncogene homolog; vJun Avian Sarcoma Virus 17 Oncogene Homolog; JUN_HUMAN; Activator 1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog.
Application Dilution
WB	=1:500-2000

Applications

Reactivity

Predicted Reactivity

Search our catalog of hundreds of thousands of products