RAB8A (8A4) Monoclonal Antibody

$Lane 1: HeLa; Lane 2: HCT116 lysate at 20 \u00b5g per lane. probed with bsm-51077M RAB8A (261CT1.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.$

Lane 1: HeLa; Lane 2: HCT116 lysate at 20 \u00b5g per lane. probed with bsm-51077M RAB8A (261CT1.3.1) Monoclonal Antibody at 1:1000 dilution and 4\u2103 overnight incubation, followed by secondary antibody incubation for 60min at room temperature.

$Lane 1: Mouse brain lysates; probed with RAB8A (261CT1.3.1) Monoclonal Antibody, unconjugated (bsm-51077M) at 1:100 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Mouse brain lysates; probed with RAB8A (261CT1.3.1) Monoclonal Antibody, unconjugated (bsm-51077M) at 1:100 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Lane 1: U937 cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Hela cell lysates probed with RAB8A (8A4) Monoclonal Antibody, Unconjugated (bsm-51077M) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51077M
Product Name	RAB8A (8A4) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This RAB8A monoclonal antibody is generated from mouse immunized with RAB8A recombinant protein.
Clonality	Monoclonal
Clone #	8A4
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	4218
Swiss Prot	P61006
Synonyms	MEL; RAB8; Ras-related protein Rab-8A; Oncogene c-mel; RAB8A
Background	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization. Plays an important role in ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis.
Application Dilution
WB	1:300-5000

Applications

Reactivity

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