PODXL (2C4) Monoclonal Antibody

$A549 cell lysates probed with PODXL (632CT5.11.4) Monoclonal Antibody, unconjugated (bsm-51201M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

A549 cell lysates probed with PODXL (632CT5.11.4) Monoclonal Antibody, unconjugated (bsm-51201M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
IHC-P
IF

Reactivity

Human

Overview
Catalog #	bsm-51201M
Product Name	PODXL (2C4) Monoclonal Antibody
Applications	WB, IHC-P, IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This PODXL antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 410-439 amino acids from the C-terminal region of human PODXL.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	5420
Swiss Prot	O00592
Synonyms	PC; PCLP; Gp200; PCLP-1; Podocalyxin; GCTM-2 antigen; Podocalyxin-like protein 1; PODXL; PCLP1
Background	Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF