H2AFX (8C5) Monoclonal Antibody

Applications

  • WB
  • IHC-P

Reactivity

  • Human
Overview
Catalog # bsm-51222M
Product Name H2AFX (8C5) Monoclonal Antibody
Applications WB, IHC-P
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.
Immunogen Range 100-143/143
Clonality Monoclonal
Clone # 8C5
Isotype IgG1
Concentration 0.5ug/ul
Purification Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3014
Swiss Prot P16104
Synonyms H2AX; H2A.X; H2A/X; Histone H2AX; Histone H2A.X; H2AFX
Background Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400