MKRN2 (56C9) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MKRN2 (56C9) Monoclonal Antibody (bsm-51326M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with MKRN2 (56C9) Monoclonal Antibody (bsm-51326M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-51326M
Product Name	MKRN2 (56C9) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This MKRN2 antibody is generated from a mouse immunized with recombinant protein.
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	23609
Swiss Prot	Q9H000
Synonyms	RNF62; HSPC070; Probable E3 ubiquitin-protein ligase makorin-2; RING finger protein 62; MKRN2
Background	E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400