PGK1 (6C1) Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded human liver tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with PGK1 (1086CT10.2.1) Monoclonal Antibody (bsm-51374M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

$Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Kidney tissue lysates; Lane 5: Mouse Testis tissue lysates; Lane 6: Mouse Liver tissue lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human MOLT4 cell lysates probed with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse NIH\/3T3 cell lysates; Lane 2: Mouse Cerebrum tissue lysates; Lane 3: Mouse Heart tissue lysates; Lane 4: Mouse Kidney tissue lysates; Lane 5: Mouse Testis tissue lysates; Lane 6: Mouse Liver tissue lysates; Lane 7: Human HeLa cell lysates; Lane 8: Human A431 cell lysates; Lane 9: Human MOLT4 cell lysates probed with PGK1 Monoclonal Antibody, Unconjugated (bsm-51374M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P

Reactivity

Human
Mouse

Overview
Catalog #	bsm-51374M
Product Name	PGK1 (6C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	This antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-417 amino acids from human.
Immunogen Range	1-417/417
Clonality	Monoclonal
Clone #	6C1
Isotype	IgG2a
Concentration	0.5ug/ul
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5230
Swiss Prot	P00558
Synonyms	PGKA; MIG10; HEL-S-68p; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; PGK1; OK/SW-cl.110
Background	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

Search our catalog of hundreds of thousands of products