| Overview |
| bsm-51396M |
| TOP1 (1C6) Monoclonal Antibody |
| WB, FCM |
| Human, Rat |
| Specifications |
| Unconjugated |
| Mouse |
| This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1. |
| Monoclonal |
| 1C6 |
| IgG1 |
| 0.5ug/ul |
| Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target |
| 7150 |
| P11387 |
| TOPI; DNA topoisomerase 1; DNA topoisomerase I; TOP1 |
| Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter. |
| Application Dilution |
| WB |
1:300-5000 |
| FCM |
1:20-100 |
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