NME1 (2C1) Monoclonal Antibody

$Sample Tissue\/Cells lysates probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51413M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.$

Sample Tissue\/Cells lysates probed with antibodyname Monoclonal Antibody, unconjugated (bsm-51413M) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at Room Temperature.

$Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NME1 (1172CT2.4.1.1) Monoclonal Antibody (bsm-51413M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human heart tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 15 minutes; Blocking buffer (3% BSA) at room temperature for 30min; Antibody incubation with NME1 (1172CT2.4.1.1) Monoclonal Antibody (bsm-51413M) at 1:25 for 1 hour at 37\u00b0C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Applications

WB
IHC-P

Reactivity

Human
Rat

Overview
Catalog #	bsm-51413M
Product Name	NME1 (2C1) Monoclonal Antibody
Applications	WB, IHC-P
Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Purified His-tagged NME1 protein was used to produced this monoclonal antibody.
Clonality	Monoclonal
Clone #	2C1
Isotype	IgG2a
Concentration	0.5ug/ul
Purification	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	4830
Swiss Prot	P15531
Synonyms	NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1; Nucleoside diphosphate kinase A; NDK A; NDP kinase A; Granzyme A-activated DNase; Metastasis inhibition factor nm23; Tumor metastatic process-associated protein; NME1
Background	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400

Applications

Reactivity

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