METAP1 Monoclonal Antibody

$Lane 1: HL-60 cell lysates; Lane 2: HepG2 cell lysates probed with METAP1 Monoclonal Antibody, Unconjugated (bsm-51490M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: HL-60 cell lysates; Lane 2: HepG2 cell lysates probed with METAP1 Monoclonal Antibody, Unconjugated (bsm-51490M) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51490m
Product Name	METAP1 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human METAP1.
Clonality	Monoclonal
Clone #	H12A1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	23173
Swiss Prot	P53582
Subcellular location	Cytoplasm
Synonyms	Methionyl Aminopeptidase 1; AMPM1_HUMAN; DKFZp781C0419; KIAA0094; MAP 1; MAP1A; MetAP-1; MetAP1; metap1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1.
Background	Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
Application Dilution
WB	=1:500-2000