TSC1 Monoclonal Antibody

$Lane 1: Mouse brain tissue lysates probed with TSC1 Monoclonal Antibody, Unconjugated (bsm-51504M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse brain tissue lysates probed with TSC1 Monoclonal Antibody, Unconjugated (bsm-51504M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Mouse

Predicted Reactivity

Human

Overview
Catalog #	bsm-51504m
Product Name	TSC1 Monoclonal Antibody
Applications	WB
Reactivity	Mouse
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human TSC1 (Fragment) (His-tagged).
Clonality	Monoclonal
Clone #	K6F13
Isotype	IgG2b
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7248
Swiss Prot	Q92574
Subcellular location	Cytoplasm, Cell membrane
Synonyms	LAM; Hamartin; Tuberous sclerosis 1 protein; TSC1_HUMAN; KIAA0243; TSC; Tuberous sclerosis 1.
Background	In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
Application Dilution
WB	=1:500-2000