C3 Monoclonal Antibody

$THP-1 (H) cells were treated with or without LPS (1\u00b5g\/ml) and BFA(Brefeldin A) (300 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with C3 monoclonal antibody, unconjugated (bsm-51535M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

THP-1 (H) cells were treated with or without LPS (1\u00b5g\/ml) and BFA(Brefeldin A) (300 ng\/ml) for 24 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with C3 monoclonal antibody, unconjugated (bsm-51535M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human

Overview
Catalog #	bsm-51535m
Product Name	C3 Monoclonal Antibody
Applications	WB
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human C3
Immunogen Range	1301-1400/1663
Clonality	Monoclonal
Clone #	B3N11
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	718
Swiss Prot	P01024
Subcellular location	Cytoplasm
Synonyms	Acylation stimulating protein cleavage product; Acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody C3 antibody C3a anaphylatoxin; CO3_HUMAN; Complement C3 alpha chain; Complement C3; Complement C3 beta chain; Complement C3 precursor; Complement C3b alpha chain; Complement C3c alpha' chain fragment 2; Complement C3c fragment; Complement C3d fragment; Complement C3f fragment; Complement C3g fragment; Complement component 3; Complement component 3 precursor; Complement component C3; Complement factor 3; CPAMD1; Plp.
Application Dilution
WB	1:500-2000