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Erk2 Monoclonal Antibody

Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NCI-H292 cell lysates; Lane 4: Hela cell lysates; Lane 5: NIH\/3T3 cell lysates; Lane 6: Neuro-2a cell lysates; Lane 7: C6 cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: MCF-7 cell lysates; Lane 2: HepG2 cell lysates; Lane 3: NCI-H292 cell lysates; Lane 4: Hela cell lysates; Lane 5: NIH\/3T3 cell lysates; Lane 6: Neuro-2a cell lysates; Lane 7: C6 cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Hela-MAPK1 KO cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Hela cell lysates; Lane 2: Hela-MAPK1 KO cell lysates probed with Erk2 Monoclonal Antibody, Unconjugated (bsm-51555M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB

Reactivity

  • Xenopus laevis

Predicted Reactivity

  • Human
  • Mouse
  • Rat
  • Cow
Overview
Catalog # bsm-51555M
Product Name Erk2 Monoclonal Antibody
Applications WB
Reactivity Xenopus laevis
Predicted Reactivity Human, Mouse, Rat, Cow
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated synthetic peptide derived from human Erk2
Immunogen Range 101-200/360
Clonality Monoclonal
Clone # D2F12
Isotype IgG1 k
Concentration 0.5ug/ul
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 5594
Swiss Prot P28482
Subcellular location Extracellular, Cytoplasm, Nucleus, Cell membrane
Synonyms ERK 2; ERK-2; ERT1; Extracellular Signal Regulated Kinase 2; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; Mapk1; Mitogen-activated protein kinase 1; MK01_HUMAN; P38; P40; P41; p42-MAPK; P42MAPK; PRKM1; PRKM2; protein kinase, mitogen-activated, 1; protein kinase, mitogen-activated, 2; protein tyrosine kinase ERK2.
Application Dilution
WB =1:500-2000，

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