GATA3 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:400 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human breast; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:400 overnight at 4\u00b0C, DAB staining.

$Lane 1: CEM cell lysates; Lane 2: HL60 cell lysates; Lane 3: SH-SY5Y cell lysates probed with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: CEM cell lysates; Lane 2: HL60 cell lysates; Lane 3: SH-SY5Y cell lysates probed with GATA3 Monoclonal Antibody, Unconjugated (bsm-51575M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt
ICC/IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51575m
Product Name	GATA3 Monoclonal Antibody
Applications	WB, Flow-Cyt, ICC/IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human GATA3.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2625
Swiss Prot	P23771
Subcellular location	Nucleus
Synonyms	GATA 3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.
Background	Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
Application Dilution
WB	=1:500-1000
Flow-Cyt	Flow-Cyt=1:25
ICC/IF	ICC/IF=1:25