CBX8 Monoclonal Antibody

$Lane 1: 293 cell lysates; Lane 2: Hela cell lysates; Lane 3: A549 cell lysates probed with CBX8 Monoclonal Antibody, Unconjugated (bsm-51593M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: 293 cell lysates; Lane 2: Hela cell lysates; Lane 3: A549 cell lysates probed with CBX8 Monoclonal Antibody, Unconjugated (bsm-51593M) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
Flow-Cyt

Predicted Reactivity

Human

Overview
Catalog #	bsm-51593m
Product Name	CBX8 Monoclonal Antibody
Applications	WB, Flow-Cyt
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	KLH conjugated synthetic peptide derived from human CBX8
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG1
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	57332
Swiss Prot	Q9HC52
Subcellular location	Nucleus
Synonyms	Chromobox protein homolog 8; HPC3; Pc3; Polycomb 3 homolog; RC1; Rectachrome 1; CBX8_HUMAN.
Background	Component of a Polycomb group (PcG) multiproteinPRC1-like complex, a complex class required to maintain thetranscriptionally repressive state of many genes, including Hoxgenes, throughout development. PcG PRC1 complex acts via chromatinremodeling and modification of histones; it mediatesmonoubiquitination of histone H2A 'Lys-119', rendering chromatinheritably changed in its expressibility.
Application Dilution
WB	=1:500-1000
Flow-Cyt	Flow-Cyt=1:25