PPP2R1B Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PPP2R1B Monoclonal Antibody, Unconjugated (bsm-51604M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with PPP2R1B Monoclonal Antibody, Unconjugated (bsm-51604M) at 1:25 overnight at 4\u00b0C, DAB staining.

$Lane 1: Jurkat cell lysates; Lane 2: Human brain tissue lysates; Lane 3: Mouse brain tissue lysates; Lane 4: Mouse lung tissue lysates; Lane 5: Rat brain tissue lysates probed with PPP2R1B Monoclonal Antibody, Unconjugated (bsm-51604M) at 1:4000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Jurkat cell lysates; Lane 2: Human brain tissue lysates; Lane 3: Mouse brain tissue lysates; Lane 4: Mouse lung tissue lysates; Lane 5: Rat brain tissue lysates probed with PPP2R1B Monoclonal Antibody, Unconjugated (bsm-51604M) at 1:4000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF
Flow-Cyt
ICC/IF

Predicted Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-51604m
Product Name	PPP2R1B Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF, Flow-Cyt, ICC/IF
Predicted Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human PPP2R1B.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5519
Swiss Prot	P30154
Subcellular location	Extracellular, Secreted
Synonyms	2AAB_HUMAN; beta isoform of regulatory subunit A, protein phosphatase 2; PP2A A beta; PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta; PP2A, subunit A, PR65 beta isoform; PP2A, subunit A, R1 beta isoform; PPP2R1B; PR65B; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), beta isoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform; Protein phosphatase 2 regulatory subunit A alpha isoform; protein phosphatase 2, structural/regulatory subunit A, beta isoform; Serine threonine protein phosphatase 65 kDa; serine/threonine protein phosphatase 2A 65 kDa regulatory subunit A, beta isoform; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, beta isoform; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform.
Background	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:25
IHC-F	IHC-F=1:25
IF	IF=1:25
Flow-Cyt	Flow-Cyt=1:25
ICC/IF	ICC/IF=1:25