MBD2 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MBD2 Monoclonal Antibody, Unconjugated (bsm-51671M) at 1:25 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with MBD2 Monoclonal Antibody, Unconjugated (bsm-51671M) at 1:25 overnight at 4\u00b0C, DAB staining.

Applications

WB
IHC-P
IHC-F
IF

Predicted Reactivity

Human

Overview
Catalog #	bsm-51671m
Product Name	MBD2 Monoclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Predicted Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Source	Recombinant human MBD2 between 10-228 amino acids.
Clonality	Monoclonal
Clone #	#REF!
Concentration	0.5ug/ul
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8932
Swiss Prot	Q9UBB5
Subcellular location	Nucleus
Synonyms	Demethylase; DMTase; MBD 2; MBD2_HUMAN; MBD2a; Methyl CpG binding domain protein 2; Methyl CpG binding protein MBD2; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY CO 41.
Background	Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Functions as a scaffold protein, targeting GATAD2A and GATAD2B to chromatin to promote repression. May enhance the activation of some unmethylated cAMP-responsive promoters.
Application Dilution
WB	=1:500-4000
IHC-P	IHC-P=1:25
IHC-F	IHC-F=1:25
IF	IF=1:25