CRISPR-Cas9 (1E8) Monoclonal Antibody

Applications

  • WB

Reactivity

  • Human
  • Others
Overview
Catalog # bsm-52042M
Product Name CRISPR-Cas9 (1E8) Monoclonal Antibody
Applications WB
Reactivity Human, Others
Specifications
Conjugation Unconjugated
Host Mouse
Source Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)
Clonality Monoclonal
Clone # 100000000
Isotype IgM
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Store at -20°C for 12 months..
Target
Gene ID 901176
Swiss Prot Q99ZW2
Synonyms CRISPR; Cas9; CRISPR-associated endonuclease Cas9/Csn1; SpyCas9; SpCas9; csn1
Background CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
Application Dilution
WB 1:300-1000