Cyclin D1 (2H4) Monoclonal Antibody

Lane 1: HeLa Cell lysates; Lane 2: PC12 Cell lysates; Lane 3: SH-SY5Y Cell lysates;probed with Cyclin D1 (2H4) Monoclonal Antibody (bsm-52046R) at 1:1000 overnight at 4˚C. Followed by a conjugated secondary antibody.

$Paraformaldehyde-fixed, paraffin embedded Human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody (bsm-52046R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37\u00b0C for 20min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody (bsm-52046R) at 1:50 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody, Unconjugated (bsm-52046R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with Cyclin D1 (2H4) Monoclonal Antibody, Unconjugated (bsm-52046R) at 1:200 overnight at 4\u00b0C, DAB staining.

Lane 1: Human MCF-7 cell lysates; Lane 2: Human HepG2 cell lysates; Lane 3: Human A549 cell lysates; Lane 4: Human U251 cell lysates probed with Cyclin D1 Monoclonal Antibody, Unconjugated (bsm-52046R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)

Reactivity

Human

Overview
Catalog #	bsm-52046R
Product Name	Cyclin D1 (2H4) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC)
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human Cyclin D1 protein, around C-terminal 150aa.
Clonality	Monoclonal
Clone #	2H4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	595
Swiss Prot	P24385
Synonyms	Cyclin D1 phospho T286; p-Cyclin D1 phospho T286; AI327039; B cell ccl/lymphoma 1; B cell leukemia 1; B-cell CLL/lymphoma 1; B-cell leukemia 1; B-cell lymphoma 1 protein; BCL-1; BCL1; BCL1 oncogene; CCND 1; CCND1; CCND1 protein; CCND1/FSTL3 fusion gene, included; CCND1/IGHG1 fusion gene; CCND1/IGHG1 fusion gene, included; CCND1/IGLC1 fusion gene, included; CCND1/PTH fusion gene, included; cD1; Cyclin D1; Cyl-1; D11S287E; G1/S specic cyclin D1; MGC137744; Parathyroid adenomatosis 1; PRAD1; PRAD1 oncogene; U21B31; CCND1_HUMAN.
Background	The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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