p53(acetyl K370) (2F1) Monoclonal Antibody

$Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours, whole cell lysates (positive control); Lane 2: untreated HepG2, whole cell lysates; probed with p53(acetyl K370) (2F1) Monoclonal Antibody, Unconjugated (bsm-52288R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.$

Lane 1: HepG2 treated with Etoposide 20uM and Trichostatin A 500 nM for 6 hours, whole cell lysates (positive control); Lane 2: untreated HepG2, whole cell lysates; probed with p53(acetyl K370) (2F1) Monoclonal Antibody, Unconjugated (bsm-52288R) at 1:1000 overnight at 4\u00b0C followed by incubation with a conjugated secondary antibody at 1:20000 for 60 minutes at 37\u00b0C.

$IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in PANC-1 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with p53(acetyl K370) (2F1) Monoclonal Antibody (bsm-52288R) at 1:100 in HepG2 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52288R
Product Name	p53(acetyl K370) (2F1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthetic peptide surrounding K370 of human p53
Modification Site	acetyl K370
Clonality	Monoclonal
Clone #	2F1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7157
Swiss Prot	P04637
Synonyms	Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53
Background	Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200

Applications

Reactivity

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