gamma Tubulin (8C1) Monoclonal Antibody

Flow cytometric analysis of Jurkat cells with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: A431 lysates; Lane 2: Jurkat lysates; Line 3: Mouse brain lysates; Probed with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A431 lysates; Lane 2: Jurkat lysates; Line 3: Mouse brain lysates; Probed with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with gamma Tubulin (8C1) Monoclonal Antibody (bsm-52814R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52814R
Product Name	gamma Tubulin (8C1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminus human gamma Tubulin
Clonality	Monoclonal
Clone #	8C1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	7283
Swiss Prot	P23258
Synonyms	Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, Tubulin gamma-2 chain, Gamma-2-tubulin, GCP-1, TUBG1, TUBG2
Background	Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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