SIRT1 (3C7) Monoclonal Antibody

$Lane 1: HepG2; Lane 2: Jurkat lysate probed with SIRT1 (3C7) Monoclonal Antibody, unconjugated (bsm-52315R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: HepG2; Lane 2: Jurkat lysate probed with SIRT1 (3C7) Monoclonal Antibody, unconjugated (bsm-52315R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in HeLa cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in NIH\/3T3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with SIRT1 (3C7) Monoclonal Antibody (bsm-52315R) at 1:100 in SH-SY5Y cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52315R
Product Name	SIRT1 (3C7) Monoclonal Antibody
Applications	WB IF(ICC) IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human SIRT1 between 700-800 amino acids
Clonality	Monoclonal
Clone #	3C7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	23411
Swiss Prot	Q96EB6
Synonyms	NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, hSIRT1, hSIR2, SIRT1
Background	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Application Dilution
WB	1:300-5000
IF(ICC)	1:50-200
IHC