BDNF (2A1) Monoclonal Antibody

Lane 1: SHG-44 cell lysates; Lane 2: A172 cell lysates; Lane 3: Mouse brain lysates probed with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

$Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human lung tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with BDNF (2A1) Monoclonal Antibody (bsm-52368R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebellum lysates; Lane 4: Rat Cerebellum lysates; Lane 5: Mouse Muscle lysates; Lane 6: Rat Muscle lysates; Lane 7: Mouse Heart lysates; Lane 8: Rat Heart lysates; Lane 9: Mouse Testis lysates; Lane 10: Rat Testis lysates; Lane 11: Human Hela cell lysates; Lane 12: Human U251 cell lysates; Lane 13: Human A549 cell lysates; Lane 14: Human U-2OS cell lysates probed with BDNF (2A1) Monoclonal Antibody, Unconjugated (bsm-52368R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
FCM
IHC-P
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52368R
Product Name	BDNF (2A1) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human BDNF between 1 to 100 amino acids
Clonality	Monoclonal
Clone #	2A1
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	627
Swiss Prot	P23560
Synonyms	Brain-derived neurotrophic factor, Abrineurin, BDNF
Background	During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IHC

Applications

Reactivity

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