GSK3 beta (1C8) Monoclonal Antibody

$Lane 1: Hela cell lysates;Lane 2: 293 cell lysates;Lane 3: NIH\/3T3 cell lysates; Lane 4: PC12 cell lysates probed with \r\nGSK3 beta (1C8 ) Monoclonal Antibody (bsm-52403R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.$

Lane 1: Hela cell lysates;Lane 2: 293 cell lysates;Lane 3: NIH\/3T3 cell lysates; Lane 4: PC12 cell lysates probed with \r\nGSK3 beta (1C8 ) Monoclonal Antibody (bsm-52403R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.

Flow cytometric analysis of Hela cells with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$IF(ICC) staining with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100 in PC-3M cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100 in SKOV-3 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse prostate tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human breast carcinoma tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse testis tissue incubated with GSK3 beta (1C8) Monoclonal Antibody (bsm-52403R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
FCM
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse

Overview
Catalog #	bsm-52403R
Product Name	GSK3 beta (1C8) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant human GSK3 beta between 350 amino acids to C-terminus
Clonality	Monoclonal
Clone #	1C8
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	2932
Swiss Prot	P49841
Subcellular location	Nucleus, Cell membrane
Synonyms	Glycogen synthase kinase-3 beta, Serine/threonine-protein kinase GSK3B, GSK3B
Background	The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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