DIABLO (9A7) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52544R |
| Product Name | DIABLO (9A7) Monoclonal Antibody |
| Applications |
WB
FCM
IHC-P
IF(ICC)
IHC
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Recombinant protein within DIABLO 200 amino acids to the C-terminus |
| Clonality | Monoclonal |
| Clone # | 9A7 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 56616 |
| Swiss Prot | Q9NR28 |
| Synonyms | Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase, Smac, DIABLO |
| Background | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IF(ICC) | 1:50-200 |
| IHC | |