NOX2/gp91phox (9B12) Monoclonal Antibody

$Lane 1: MCF-7 Cells; Lane 2: THP-1 Cells; Lane 3: HepG2 Cells; Probed with NOX2\/gp91phox (9B12) Monoclonal Antibody (bsm-52603R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: MCF-7 Cells; Lane 2: THP-1 Cells; Lane 3: HepG2 Cells; Probed with NOX2\/gp91phox (9B12) Monoclonal Antibody (bsm-52603R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates probed with NOX2\/gp91phox Monoclonal Antibody, Unconjugated (bsm-52603R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human A549 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human MCF-7 cell lysates probed with NOX2\/gp91phox Monoclonal Antibody, Unconjugated (bsm-52603R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52603R
Product Name	NOX2/gp91phox (9B12) Monoclonal Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human NOX2/gp91phox aa 150-250
Clonality	Monoclonal
Clone #	9B12
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	1536
Swiss Prot	P04839
Synonyms	Cytochrome b-245 heavy chain, CGD91-phox, Cytochrome b(558) subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase 2, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-geneRating NADPH oxidase heavy chain subunit, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, Cytochrome b558 subunit beta, CYBB
Background	Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Application Dilution
WB

Applications

Reactivity

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