PP2A alpha + beta (11F10) Monoclonal Antibody

$Lane 1: A431 Cells; Lane 2: NIH\/3T3 Cells; Lane 3: 293T Cells; Probed with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: A431 Cells; Lane 2: NIH\/3T3 Cells; Lane 3: 293T Cells; Probed with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in 293 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human pancreas tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100 in L6 cells (green). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat kidney tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse stomach tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse heart tissue incubated with PP2A alpha + beta (11F10) Monoclonal Antibody (bsm-52611R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat
Zebrafish

Overview
Catalog #	bsm-52611R
Product Name	PP2A alpha + beta (11F10) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human, Mouse, Rat, Zebrafish
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human PP2A alpha + beta aa 250-350
Clonality	Monoclonal
Clone #	11F10
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Swiss Prot	P62714, P67775
Synonyms	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-alpha, RP-C, PPP2CA, PP2A-beta, PPP2CB
Background	The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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