Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-52695R |
| Product Name | Ubiquitin-like modifier-activating enzyme 1 (8A4) Monoclonal Antibody |
| Applications |
WB
FCM
IHC-P
IF(ICC)
IHC
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | Human Ubiquitin-like modifier-activating enzyme 1 between 1000 amino acids to C-terminus |
| Clonality | Monoclonal |
| Clone # | 8A4 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 7317 |
| Swiss Prot | P22314 |
| Synonyms | Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1, UBA1 |
| Background | Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IF(ICC) | 1:50-200 |
| IHC | |