THY1 (4C6) Monoclonal Antibody

$Lane 1: Hela lysates; Lane 2: HepG2 cells lysates; Line 3: HUVEC lysates; Probed with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Hela lysates; Lane 2: HepG2 cells lysates; Line 3: HUVEC lysates; Probed with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:1000 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

$Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human kidney tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human lung cancer tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Human tonsil tissue incubated with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100 in Hela cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with THY1 (4C6) Monoclonal Antibody (bsm-52869R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffinembedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:50\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52869R$

Tissue: Human cerebrum\r\nSection type: Formalin-fixed & Paraffinembedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:50\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52869R

$Tissue: Human colon cancer\r\nSection type: Formalin-fixed & Paraffinembedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:50\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52869R$

Tissue: Human colon cancer\r\nSection type: Formalin-fixed & Paraffinembedded section\r\nRetrieval method: High temperature and high pressure\r\nRetrieval buffer: Tris\/EDTA buffer, pH 9.0\r\nPrimary Ab dilution: 1:50\r\nPrimary Ab incubation condition: 1 hour at room temperature\r\nSecondary Ab: SP Kit(Rabbit) (sp-0023)\r\nCounter stain: Hematoxylin (Blue)\r\nComment: Color brown is the positive signal for bsm-52869R

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Thymus tissue lysates probed with CD90\/Thy-1 Monoclonal Antibody, Unconjugated (bsm-52869R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Thymus tissue lysates probed with CD90\/Thy-1 Monoclonal Antibody, Unconjugated (bsm-52869R) at 1:2000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human

Overview
Catalog #	bsm-52869R
Product Name	THY1 (4C6) Monoclonal Antibody
Applications	WB, IHC-P, IF(ICC), IHC
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	N terminal human THY1
Clonality	Monoclonal
Clone #	4C6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	7070
Swiss Prot	P04216
Synonyms	Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD_antigen: CD90, THY1
Background	May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC

Applications

Reactivity

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