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TNF Receptor II (7D3) Monoclonal Antibody

Flow cytometric analysis of HL-60 cells with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Flow cytometric analysis of HL-60 cells with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black)
Lane 1: MCF-7 cells lysates; Lane 2: Jurkat cells lysates; Probed with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
Lane 1: MCF-7 cells lysates; Lane 2: Jurkat cells lysates; Probed with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.
IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in MCF-7 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
IF(ICC) staining with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100 in SW480 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Paraformaldehyde-fixed and paraffin-embedded Human uterus tissue incubated with TNF Receptor II (7D3) Monoclonal Antibody (bsm-52938R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.
Lane 1: Human THP-1 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U937 cell; Lane 4: Human U87MG cell\uff1bLane 5: Human HUVEC cell. Probed with TNFR2 monoclonal Antibody, unconjugated (bsm-52938R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human THP-1 cell lysates; Lane 2: Human K562 cell lysates; Lane 3: Human U937 cell; Lane 4: Human U87MG cell\uff1bLane 5: Human HUVEC cell. Probed with TNFR2 monoclonal Antibody, unconjugated (bsm-52938R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

  • WB
  • FCM
  • IHC-P
  • IF(ICC)
  • IHC

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-52938R
Product Name TNF Receptor II (7D3) Monoclonal Antibody
Applications WB, FCM, IHC-P, IF(ICC), IHC
Reactivity Human, Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source C terminus Human TNF Receptor II
Clonality Monoclonal
Clone # 7D3
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7133
Swiss Prot P20333
Synonyms Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b, Tumor necrosis factor receptor superfamily member 1b, membrane form, Tumor necrosis factor-binding protein 2, TNF-R2, TNF-RII, TNFR-II, TNFRSF1B
Background Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400
IF(ICC) 1:50-200
IHC

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