FMRP (1B6) Monoclonal Antibody

$Lane 1: Hela cells lysates; Lane 2: K562 cells lysates; Probed with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n\r\n$

Lane 1: Hela cells lysates; Lane 2: K562 cells lysates; Probed with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n\r\n\r\n

$Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse colon tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:200 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:200 in HepG2 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:200 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with FMRP (1B6) Monoclonal Antibody (bsm-52945R) at 1:200 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
IHC-P
IF(ICC)
IHC

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52945R
Product Name	FMRP (1B6) Monoclonal Antibody
Applications	WB IHC-P IF(ICC) IHC
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Human FMRP aa 1-100
Clonality	Monoclonal
Clone #	1B6
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20C for 12 months.
Target
Gene ID	2332
Swiss Prot	Q06787
Synonyms	Synaptic functional regulator FMR1, Fragile X mental retardation protein 1, FMRP, Protein FMR-1, FMR1
Background	Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression (By similarity). RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. May play a role in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C).
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(ICC)	1:50-200
IHC