NMDAR1 (13C4) Monoclonal Antibody

$Lane 1: MCF-7 cells lysates; Lane 2: A549 cells lysates; Probed with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n$

Lane 1: MCF-7 cells lysates; Lane 2: A549 cells lysates; Probed with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.\r\n

Flow cytometric analysis of sh-sy5y cells with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Mouse brain tissue incubated with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.$

Paraformaldehyde-fixed and paraffin-embedded Rat brain tissue incubated with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:100, overnight at 4\u00b0C, followed by a conjugated secondary antibody and DAB staining. Counterstained with hematoxylin.

$IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R at 1:100 in N2A cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:200 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:200 in SHG-44 cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

$IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:200 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.$

IF(ICC) staining with NMDAR1 (13C4) Monoclonal Antibody (bsm-52950R) at 1:200 in SH-SY5Y cells (red). The nuclear counterstain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100\/PBS.

Applications

WB
FCM
IHC-P
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-52950R
Product Name	NMDAR1 (13C4) Monoclonal Antibody
Applications	WB, FCM, IHC-P, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	C terminus human NMDAR1
Clonality	Monoclonal
Clone #	13C4
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2902
Swiss Prot	Q05586
Synonyms	Glutamate receptor ionotropic, NMDA 1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, GluN1, NMD-R1
Background	NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400
IF(ICC)	1:50-200

Applications

Reactivity

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