KAT2B (2C1) Monoclonal Antibody

Flow cytometric analysis of Hela cells with KAT2B (2C1) Monoclonal Antibody (bsm-54229R) 1:100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

$Lane 1: Rat Kidney; Lane 2: K562 Cells; Probed with KAT2B (2C1) Monoclonal Antibody (bsm-54229R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.$

Lane 1: Rat Kidney; Lane 2: K562 Cells; Probed with KAT2B (2C1) Monoclonal Antibody (bsm-54229R) at 1:500 overnight at 4\u00b0C followed by a conjugated secondary antibody for 60 minutes at 37\u00b0C.

Applications

WB
FCM
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-54229R
Product Name	KAT2B (2C1) Monoclonal Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant protein within human PCAF aa 100-300
Clonality	Monoclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Store at 4C for up to 2 weeks. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Target
Gene ID	8850
Swiss Prot	Q92831
Subcellular location	Nucleus
Synonyms	Histone acetyltransferase KAT2B; Histone acetyltransferase PCAF; Lysine acetyltransferase 2B; P300/CBP-associated factor; Histone acetylase PCAF; P/CAF; KAT2B; PCAF.
Background	Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY, PLK4 and TBX5. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5. Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4. Also acetylates spermidine.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IF(ICC)	1:50-200