SAA4 Monoclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with SAA4 Monoclonal Antibody, Unconjugated (bsm-54660R) at 1:50 for 30 minutes at room temperature, DAB staining.

$Flow cytometric analysis of SAA4 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54660R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).$

Flow cytometric analysis of SAA4 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (bsm-54660R, 1\/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1\/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Applications

WB
FCM
IHC-P

Reactivity

Human

Overview
Catalog #	bsm-54660R
Product Name	SAA4 Monoclonal Antibody
Applications	WB, FCM, IHC-P
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Synthetic peptide within C-terminal Human SAA4.
Clonality	Monoclonal
Clone #	4F7
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide.
Storage Condition	Store at -20°C for 12 months.
Target
Gene ID	6291
Swiss Prot	P35542
Subcellular location	Secreted
Synonyms	Amyloid A, serum, 4 antibody, C SAA antibody, C-SAA antibody, Constitutively expressed serum amyloid A protein antibody, CSAA antibody, SAA 4 antibody, Saa4 antibody, SAA4 protein antibody, SAA4_HUMAN antibody, Serum amyloid A 4 protein antibody, Serum amyloid A-4 protein antibody, Serum amyloid A4 constitutive antibody
Background	SAA4 is a constitutively expressed protein belonging to the SAA family. It is a major acute phase reactant and an apolipoprotein of the HDL complex. The serum amyloid A (SAA) superfamily comprises a number of differentially expressed genes with a high degree of homology in mammalian species. SAA4, an apolipoprotein constitutively expressed only in humans and mice, is associated almost entirely with lipoproteins of the high density range. Its physiological function is unknown and its serum concentration has no relationship with those of other major apolipoproteins. The presence of SAA4 mRNA and protein in macrophage derived foam cells of coronary and carotid arteries suggested a specific role of human SAA4 during inflammation including atherosclerosis.
Application Dilution
WB	1:300-5000
FCM	1:20-100
IHC-P	1:200-400

Applications

Reactivity

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